No. But back when Nie was the only one who'd messed with the room so far, I asked Blue if we should worry about him, and they said only if he did it again.
So while it's not ideal to lose any part of your soul... at least individually, the amount lost should be trivial enough to not impact you.
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Though it's not as if gods are exempt to penalties on the soul, so it's not completely out of the question.
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[ extremely yikes. ]
Does it... feel different? To be missing it?
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No. But back when Nie was the only one who'd messed with the room so far, I asked Blue if we should worry about him, and they said only if he did it again.
So while it's not ideal to lose any part of your soul... at least individually, the amount lost should be trivial enough to not impact you.